Cytotoxic Effect of Prunus Divaricata Ledeb. Extract on Lung Cancer Cells and Determination of Expression Profiles of Genes in the MAP-Kinase Pathway

Cherry plum (Prunus divaricata Ledeb.) is a diploid fruit tree that belongs to the Rosaceae family. Anticarcinogenic activity of Prunus Ledeb. methanol extract was examined in lung cancer cell lines A549 and H1299 as well healthy line Beas-2b this study. The changes expression levels most frequently mutated oncogenes cancer, KRAS, EGFR, BRAF, PIK3CA were investigated well. In vitro cytotoxicity assays P. conducted by measuring percentage viable cells spectrophotometrically using viability identification kit (CVDK) based on colorimetric method. AB 7500 Fast RT-PCR device with Sybr Green Master Mix used create gene profiles, Ct values all genes assessed normalizing value housekeeping gene. found have cytotoxic effect at 20 mg/ml, but not these concentrations. By reducing proliferation, low KRAS caused undergo apoptosis. has been inhibit development cancerous while promoting proliferation cells. We believe it does through apoptotic pathway regulating via MAP-Kinase pathway.